Successful Marketing Strategies for Promoting Clinical Diagnostic Instrument Validation Packages

Successful marketing strategies for clinical validation packages not only help laboratories increase the accuracy and efficiency of testing, but also facilitate clinical quality awareness and collaboration. False diagnosis and inefficiency in healthcare can be costly, and managers in diagnostic instrument manufacturing organizations need strategies to promote validation packages to help laboratories reduce errors leading to inappropriate treatment. The purpose of this single case study was to explore strategies business development managers use to integrate dynamic capabilities for marketing instrument validation packages aimed to increase clinical laboratory quality and test accuracy. The conceptual framework was Teece\u27s concept of dynamic capabilities. The data collection process involved semistructured interviews with 4 business development managers from a diagnostic instrument manufacturing organization in the western United States who had successfully marketed validation packages. Analysis of the audio recordings, notes from the interviews, and marketing flyers yielded 1 overarching theme, collaboration of cross-functional teams, and 4 subthemes: integration, effectiveness, partnership, and profitability. The results suggested dynamic capabilities created value for validation packages, differentiated the products and services from those from the competitors, and increased customer satisfaction and profitability. The implications for positive social change include the potential to promote validation packages to clinical laboratories and raise awareness of laboratory quality, leading to improved healthcare outcomes

Identification of novel DNA methylation inhibitors via a two-component reporter gene system

<p>Abstract</p> <p>Background</p> <p>Targeting abnormal DNA methylation represents a therapeutically relevant strategy for cancer treatment as demonstrated by the US Food and Drug Administration approval of the DNA methyltransferase inhibitors azacytidine and 5-aza-2'-deoxycytidine for the treatment of myelodysplastic syndromes. But their use is associated with increased incidences of bone marrow suppression. Alternatively, procainamide has emerged as a potential DNA demethylating agent for clinical translation. While procainamide is much safer than 5-aza-2'-deoxycytidine, it requires high concentrations to be effective in DNA demethylation in suppressing cancer cell growth. Thus, our laboratories have embarked on the pharmacological exploitation of procainamide to develop potent DNA methylation inhibitors through lead optimization.</p> <p>Methods</p> <p>We report the use of a DNA methylation two-component enhanced green fluorescent protein reporter system as a screening platform to identify novel DNA methylation inhibitors from a compound library containing procainamide derivatives.</p> <p>Results</p> <p>A lead agent IM25, which exhibits substantially higher potency in <it>GSTp1 </it>DNA demethylation with lower cytotoxicity in MCF7 cells relative to procainamide and 5-aza-2'-deoxycytidine, was identified by the screening platform.</p> <p>Conclusions</p> <p>Our data provide a proof-of-concept that procainamide could be pharmacologically exploited to develop novel DNA methylation inhibitors, of which the translational potential in cancer therapy/prevention is currently under investigation.</p

Pervasive brain monitoring and data sharing based on multi-tier distributed computing and linked data technology

EEG-based Brain-computer interfaces (BCI) are facing grant challenges in their real-world applications. The technical difficulties in developing truly wearable multi-modal BCI systems that are capable of making reliable real-time prediction of users’ cognitive states under dynamic real-life situations may appear at times almost insurmountable. Fortunately, recent advances in miniature sensors, wireless communication and distributed computing technologies offered promising ways to bridge these chasms. In this paper, we report our attempt to develop a pervasive on-line BCI system by employing state-of-art technologies such as multi-tier fog and cloud computing, semantic Linked Data search and adaptive prediction/classification models. To verify our approach, we implement a pilot system using wireless dry-electrode EEG headsets and MEMS motion sensors as the front-end devices, Android mobile phones as the personal user interfaces, compact personal computers as the near-end fog servers and the computer clusters hosted by the Taiwan National Center for High-performance Computing (NCHC) as the far-end cloud servers. We succeeded in conducting synchronous multi-modal global data streaming in March and then running a multi-player on-line BCI game in September, 2013. We are currently working with the ARL Translational Neuroscience Branch and the UCSD Movement Disorder Center to use our system in real-life personal stress and in-home Parkinson’s disease patient monitoring experiments. We shall proceed to develop a necessary BCI ontology and add automatic semantic annotation and progressive model refinement capability to our system

Women with endometriosis have higher comorbidities: Analysis of domestic data in Taiwan

AbstractEndometriosis, defined by the presence of viable extrauterine endometrial glands and stroma, can grow or bleed cyclically, and possesses characteristics including a destructive, invasive, and metastatic nature. Since endometriosis may result in pelvic inflammation, adhesion, chronic pain, and infertility, and can progress to biologically malignant tumors, it is a long-term major health issue in women of reproductive age. In this review, we analyze the Taiwan domestic research addressing associations between endometriosis and other diseases. Concerning malignant tumors, we identified four studies on the links between endometriosis and ovarian cancer, one on breast cancer, two on endometrial cancer, one on colorectal cancer, and one on other malignancies, as well as one on associations between endometriosis and irritable bowel syndrome, one on links with migraine headache, three on links with pelvic inflammatory diseases, four on links with infertility, four on links with obesity, four on links with chronic liver disease, four on links with rheumatoid arthritis, four on links with chronic renal disease, five on links with diabetes mellitus, and five on links with cardiovascular diseases (hypertension, hyperlipidemia, etc.). The data available to date support that women with endometriosis might be at risk of some chronic illnesses and certain malignancies, although we consider the evidence for some comorbidities to be of low quality, for example, the association between colon cancer and adenomyosis/endometriosis. We still believe that the risk of comorbidity might be higher in women with endometriosis than that we supposed before. More research is needed to determine whether women with endometriosis are really at risk of these comorbidities

Isolation and Characterization of Novel Murine Epiphysis Derived Mesenchymal Stem Cells

BACKGROUND: While bone marrow (BM) is a rich source of mesenchymal stem cells (MSCs), previous studies have shown that MSCs derived from mouse BM (BMMSCs) were difficult to manipulate as compared to MSCs derived from other species. The objective of this study was to find an alternative murine MSCs source that could provide sufficient MSCs. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we described a novel type of MSCs that migrates directly from the mouse epiphysis in culture. Epiphysis-derived MSCs (EMSCs) could be extensively expanded in plastic adherent culture, and they had a greater ability for clonogenic formation and cell proliferation than BMMSCs. Under specific induction conditions, EMSCs demonstrated multipotency through their ability to differentiate into adipocytes, osteocytes and chondrocytes. Immunophenotypic analysis demonstrated that EMSCs were positive for CD29, CD44, CD73, CD105, CD166, Sca-1 and SSEA-4, while negative for CD11b, CD31, CD34 and CD45. Notably, EMSCs did not express major histocompatibility complex class I (MHC I) or MHC II under our culture system. EMSCs also successfully suppressed the proliferation of splenocytes triggered by concanavalin A (Con A) or allogeneic splenocytes, and decreased the expression of IL-1, IL-6 and TNF-α in Con A-stimulated splenocytes suggesting their anti-inflammatory properties. Moreover, EMSCs enhanced fracture repair, ameliorated necrosis in ischemic skin flap, and improved blood perfusion in hindlimb ischemia in the in vivo experiments. CONCLUSIONS/SIGNIFICANCES: These results indicate that EMSCs, a new type of MSCs established by our simple isolation method, are a preferable alternative for mice MSCs due to their better growth and differentiation potentialities

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

The genetic architecture of the human cerebral cortex

The cerebral cortex underlies our complex cognitive capabilities, yet little is known about the specific genetic loci that influence human cortical structure. To identify genetic variants that affect cortical structure, we conducted a genome-wide association meta-analysis of brain magnetic resonance imaging data from 51,665 individuals. We analyzed the surface area and average thickness of the whole cortex and 34 regions with known functional specializations. We identified 199 significant loci and found significant enrichment for loci influencing total surface area within regulatory elements that are active during prenatal cortical development, supporting the radial unit hypothesis. Loci that affect regional surface area cluster near genes in Wnt signaling pathways, which influence progenitor expansion and areal identity. Variation in cortical structure is genetically correlated with cognitive function, Parkinson's disease, insomnia, depression, neuroticism, and attention deficit hyperactivity disorder

Employing Student Workers in the Hotel Industry on a Part-Time Basis

Using student workers can be seen as a flexible option for many organizations. Drawing data from interviews with hotel managers, this study aims to explore flexible options as a response to labour shortages in the Taiwan hotel industry, specifically to explore the potential of employing full time college/university students as part time workers in the hotel industry and to provide insights into hotel managers’ perceptions in employing full time college/university students to work on a part time basis. The findings suggest that student labour is considered an effective and a flexible human resource to meet employers’ needs in solving the labour shortage problem in the hotel industry. This research attempts to bridge a research gap relating to the role of student labour in the Taiwanese hotel industry, one that has been neglected in labour market research, particularly with respect to flexible labour

從「通訊傳播管理法草案」探討電信價格管制之政策方向 Some Reflections on Telecommunications Pricing Policy –With Comments on the Draft of Communications Regulatory Act”

電信資費管制主要可分為管制業者間通訊服務之「批發價格管制」及提供消費者電信服務之「零售價格管制」兩大類。其中「批發價格管制」係自維持產業競爭公平性為中心，管制目的在於防杜市場主導業者有濫用其顯著市場力量而限制市場競爭之行為。「零售價格管制」之主要目的在於，在電信市場處於獨占或缺乏競爭的時候，避免資費訂價過高，以獲取不合理的獨占利益。 我國電信事業主管機關NCC 基於通訊傳播產業匯流趨勢而著手草擬 「通訊傳播管理法草案」，當為檢視具爭議之電信價格管制措施的適當時機。本文擬自管制目的為出發，並參考國外管制經驗後，對我國現行法規及「通訊傳播管理法」中對於電信價格管制之政策進行檢視與建議。 Price regulation in the telecommunications industry can be divided into two categories－“wholesale price control” and “retail price control”. By wholesale price control, it forces dominant telecommunications carriers to set a wholesale price for providing telecommunications services to other telecommunications carriers. Retail price control requires, where there is a monopoly or a lack of effective competition in the market, certain telecommunications carriers to set reasonable and affordable prices to their ultimate consumers. In the light of the rising trend of convergence in communications sectors, Taiwan’s National Communications Commission (NCC) has finalized the draft of “Communications Regulatory Act” in 2008. By looking upon the regulatory policy and schemes of price regulation in the U.K. and U.S., the article offers some significant reflections of price regulatory reform as well as some comments with respect to pricing provisions in the new Draft